Antibody was affinity purified using an epitope specific to CCAR1 immobilized on solid support. The epitope recognized by A300-270A maps to a region between residue 700 and 750 of human Cell-Cycle and Apoptosis Regulatory Protein 1 using the numbering given in entry NP_060707.2 (GeneID 55749). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
- Applications: WB, IP
CARP-1, cell cycle and apoptosis regulatory protein 1, cell division cycle and apoptosis regulator protein 1, death inducer with SAP domain
between 700 and 750
2 - 8°C
Detection of human CCAR1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CCAR1 antibody A300-270A (lot A300-270A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.