Antibody was affinity purified using an epitope specific to FLJ20530/KAONASHI1 immobilized on solid support. The epitope recognized by A300-269A maps to a region between residues 200 and 250 of human FLJ20530/KAONASHI1 using the numbering given in TrEMBL entry Q7Z314 (GeneID 55656). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
C8orf52, INT8, integrator complex subunit 8, protein kaonashi-1
between 200 and 250
2 - 8°C
Detection of human FLJ20530/KAONASHI1 by western blot. Samples: Nuclear extract (NE) from HeLa cells. Antibody: Affinity purified rabbit anti-FLJ20530/KAONASHI1 antibody (Cat. No. A300-269A) used at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 20 minutes.
Detection of human FLJ20530/KAONASHI1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- FLJ20530/KAONASHI1 (Cat. No. A300-269A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB