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Rabbit anti-Claspin Antibody, Affinity Purified - A300-266A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Claspin Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-266A
Name:
Rabbit anti-Claspin Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Claspin immobilized on solid support. The epitope recognized by A300-266A maps to a region between residues 725 and 775 of human Claspin using the numbering given in entry AAG24515.1 (GeneID 63967).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
claspin, claspin homolog, hClaspin
Species Reactivity:
Human
Immunogen:
Between 725 and 775
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Claspin by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody A300-266A (lot A300-266A-4) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human Claspin by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody A300-266A (lot A300-266A-4) used for IP at 6 µg/mg lysate. For blotting immunoprecipitated Claspin, A300-266A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human Claspin by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Claspin (Cat. No. A300-266A Lot4) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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