Antibody was affinity purified using an epitope specific to Claspin immobilized on solid support. The epitope recognized by A300-266A maps to a region between residues 725 and 775 of human Claspin using the numbering given in entry AAG24515.1 (GeneID 63967). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
claspin, claspin homolog, hClaspin
between 725 and 775
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human Claspin by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Claspin antibody A300-266A (lot A300-266A-4) used for IP at 6 µg/mg lysate. For blotting immunoprecipitated Claspin, A300-266A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.