Antibody was affinity purified using an epitope specific to Claspin immobilized on solid support. The epitope recognized by A300-265A maps to a region between residues 450 and 500 of human Claspin using the numbering given in entry AAG24515.1 (GeneID 63967). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
claspin, claspin homolog, hClaspin
between 450 and 500
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human Claspin by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Claspin antibody A300-265A (lot A300-265A-1) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.