Antibody was affinity purified using an epitope specific to SAH3 immobilized on solid support. The epitope recognized by A300-262A maps to a region between residues 50 and 100 of human adenosylhomocysteinase 3 (KIAA0828) using the numbering given in entry NP_056143.1 (GeneID 23382). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
adenosylhomocysteinase 3, AdoHcyase 3, ADOHCYASE3, IP(3)Rs binding protein released with IP(3) 2, IRBIT2, long-IRBIT, putative adenosylhomocysteinase 3, S-adenosylhomocysteine hydrolase-like 2, S-adenosylhomocysteine hydrolase-like protein 2, S-adenosyl-L
between 50 and 100
2 - 8°C
Detection of human SAH3 by western blot and immunoprecipitation. Samples: NETN whole cell lysate (20 µg for WB; 1 mg for IP) from HeLa cells. Antibody: Affinity purified rabbit anti-SAH3 antibody (Cat. No. A300-262A) used at the indicated concentration for WB and 2 µg/mg lysate for IP. Detection: HRP conjugated goat anti-rabbit IgG (Cat. No. A120-101P) used at 1:10,000 followed by a chemiluminescent substrate and exposure to film for 10 seconds.