Antibody was affinity purified using an epitope specific to PNK1 immobilized on solid support. The epitope recognized by A300-257A maps to a region between residues 100 and 150 of human polynucleotide kinase 3-prime-phosphatase using the numbering given in TrEMBL entry Q96T60 (GeneID 11284). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
2'(3')-polynucleotidase, AOA4, bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, EIEE10, Homo sapiens polynucleotide kinase 3'-phosphatase (PNKP), MCSZ, PNK, Polynucleotide 3'-phosphatase, Polynucleotide 5'-hydroxyl-kinase, Pol
between 100 and 150
2 - 8°C
Detection of human PNK1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PNK1 antibody A300-257A (lot A300-257A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.