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Rabbit anti-Fen1 Antibody, Affinity Purified

Catalogue number:
A300-256A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to Fen1 immobilized on solid support. The epitope recognized by A300-256A maps to a region between residues 250 and 300 of human Flap Structure Endonuclease 1 using the numbering given in entry NP_004102.1 (GeneID 2237). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Product Type:

Antibodies

Alternative Names:

DNase IV, FEN-1, flap endonuclease 1, Flap structure-specific endonuclease 1, hFEN-1, Maturation factor 1, maturation factor-1, MF1, RAD2

Species Reactivity:

Human

Immunogen:

between 250 and 300

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Fen1 by western blot.

Detection of human Fen1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Fen1 antibody A300-256A (lot A300-256A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Fen1 by western blot

Detection of human Fen1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Fen1 antibody A300-256A (lot A300-256A-2) used for IP at 6 µg per reaction. Fen1 was also immunoprecipitated by a previous lot of this antibody (lot A300-256A-1). For blotting immunoprecipitated Fen1, A300-256A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Fen1 by immunohistoch

Detection of human Fen1 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Fen1 (Cat. No. A300-256A Lot2) used at a dilution of 1:1000 (1µg/ml). Detection: DAB