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  2. Bethyl Laboratories, Inc.
  3. Rabbit anti-PLK1 Antibody, Affinity Purified
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Rabbit anti-PLK1 Antibody, Affinity Purified

Catalogue number:
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A300-251A
Supplier:
Bethyl Laboratories, Inc.
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
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£436.00
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Estimated delivery Wed 24 Apr
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Applications:

WB, IP, ICC-IF

Antibody Host:

Rabbit

Target:

PLK1

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

between 325 and 375

Alternative Names:

PLK; serine/threonine-protein kinase 13; STPK13; serine/threonine-protein kinase PLK1; cell cycle regulated protein kinase; PLK-1; Polo-like kinase 1; polo (Drosophia)-like kinase

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Product Description:

Rabbit anti-PLK1 Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human PLK1 by western blot.
Detection of human PLK1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody A300-251A (lot A300-251A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human PLK1 by immunocytoche
Detection of human PLK1 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- PLK1 (Cat. No.A300-251A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: Green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated (Cat. No. A120-501F) used at a dilution of 1:100.
Detection of human PLK1 by western blot
Detection of human PLK1 by western blot and immunoprecipitation. Samples: Whole cell lysate (50 µg for A; 600 µg for B) from HeLa cells. Antibodies: A. Affinity purified rabbit anti-PLK1 antibody A300-251A used at the indicated concentrations for WB. B. Affinity purified rabbit anti-PLK1 antibodies A300-250A, A300-251A, and BL978 used at 2 µg/600 µg lysate for IP. immunoprecipitatesd PLK1 was detected using BL978 at 0.5 µg/ml. Detection: Chemiluminescence with exposure times of less than 5 minutes.
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