Antibodies were affinity purified using epitopes specific to PLK1 immobilized on solid support. The epitopes recognized by A300-250A maps to a region between residues 1 and 50 of human Polo-like kinase 1 using the numbering given in SwissProt entry P53350 (GeneID 5347). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
cell cycle regulated protein kinase, PLK, PLK-1, polo (Drosophia)-like kinase, Polo-like kinase 1, serine/threonine-protein kinase 13, serine/threonine-protein kinase PLK1, STPK13
between 1 and 50
2 - 8°C
Detection of human PLK1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody A300-250A (lot A300-250A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human PLK1 by western blot and immunoprecipitation. Samples: Whole cell lysate (50 µg for A; 600 µg for B) from HeLa cells. Antibodies: A. Affinity purified rabbit anti-PLK1 antibody A300-250A used at the indicated concentrations for WB. B. Affinity purified rabbit anti-PLK1 antibodies A300-250A, A300-251A, and BL978 used at 2 µg/600 µg lysate for IP. Immunoprecipitatesd PLK1 was detected using BL978 at 0.5 µg/ml. Detection: Chemiluminescence with exposure times of less than 5 minutes.