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Rabbit anti-PLK1 Antibody, Affinity Purified - A300-250A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-PLK1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-250A
Name:
Rabbit anti-PLK1 Antibody, Affinity Purified
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Availability:

Antibodies were affinity purified using epitopes specific to PLK1 immobilized on solid support. The epitopes recognized by A300-250A maps to a region between residues 1 and 50 of human Polo-like kinase 1 using the numbering given in SwissProt entry P53350 (GeneID 5347).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
cell cycle regulated protein kinase, PLK, PLK-1, polo (Drosophia)-like kinase, Polo-like kinase 1, serine/threonine-protein kinase 13, serine/threonine-protein kinase PLK1, STPK13
Species Reactivity:
Human
Immunogen:
Between 1 and 50
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human PLK1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody A300-250A (lot A300-250A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human PLK1 by western blot and immunoprecipitation. Samples: Whole cell lysate (50 µg for A; 600 µg for B) from HeLa cells. Antibodies: A. Affinity purified rabbit anti-PLK1 antibody A300-250A used at the indicated concentrations for WB. B. Affinity purified rabbit anti-PLK1 antibodies A300-250A, A300-251A, and BL978 used at 2 µg/600 µg lysate for IP. Immunoprecipitatesd PLK1 was detected using BL978 at 0.5 µg/ml. Detection: Chemiluminescence with exposure times of less than 5 minutes.

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