www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

Rabbit anti-PLK1 Antibody, Affinity Purified

Catalogue number:
A300-250A
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£322.00 Shipping is calculated in checkout

Antibodies were affinity purified using epitopes specific to PLK1 immobilized on solid support. The epitopes recognized by A300-250A maps to a region between residues 1 and 50 of human Polo-like kinase 1 using the numbering given in SwissProt entry P53350 (GeneID 5347). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

cell cycle regulated protein kinase, PLK, PLK-1, polo (Drosophia)-like kinase, Polo-like kinase 1, serine/threonine-protein kinase 13, serine/threonine-protein kinase PLK1, STPK13

Species Reactivity:

Human, Mouse

Immunogen:

between 1 and 50

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human PLK1 by western blot.

Detection of human PLK1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PLK1 antibody A300-250A (lot A300-250A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human PLK1 by western blot

Detection of human PLK1 by western blot and immunoprecipitation. Samples: Whole cell lysate (50 µg for A; 600 µg for B) from HeLa cells. Antibodies: A. Affinity purified rabbit anti-PLK1 antibody A300-250A used at the indicated concentrations for WB. B. Affinity purified rabbit anti-PLK1 antibodies A300-250A, A300-251A, and BL978 used at 2 µg/600 µg lysate for IP. Immunoprecipitatesd PLK1 was detected using BL978 at 0.5 µg/ml. Detection: Chemiluminescence with exposure times of less than 5 minutes.