Rabbit anti-p53 Antibody, Affinity Purified - A300-248A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to p53 immobilized on solid support. The epitope recognized by A300-248A maps to a region between residues 325 and 375 of human tumor protein p53 using the numbering given in SwissProt entry P04637 (GeneID 7157). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP
Detection of human p53 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p53 antibody A300-248A (lot A300-248A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human p53 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-p53 antibody A300-248A (lot A300-248A-2) used for IP at 6 µg per reaction. p53 was also immunoprecipitated by a previous lot of this antibody (lot A300-248A-1). For blotting immunoprecipitated p53, A300-248A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
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