Rabbit anti-p53 Antibody, Affinity Purified

Name: Rabbit anti-p53 Antibody, Affinity Purified
Brand: Bethyl Laboratories, Inc.
SKU: CBSKU_00070545
Price: 436 GBP
Availability: InStock

Catalogue number:

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A300-247A

Supplier:

Bethyl Laboratories, Inc.

Size:

100 µl (1000 µg/ml) _$$_

Product is available in:

Currency:
GBP
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£436.00

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Estimated delivery Wed 1 May
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Applications:

WB, IP, IHC, ChIP-Seq, Flow Cyt

Antibody Host:

Rabbit

Target:

p53

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

between 50 and 100

Alternative Names:

phosphoprotein p53; tumor supressor p53; Tumor suppressor p53; tumor protein 53; TRP53; transformation-related protein 53; P53; mutant tumor protein 53; LFS1; cellular tumor antigen p53; BCC7; antigen NY-CO-13; p53 tumor suppressor

Product Description:

Rabbit anti-p53 Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human P53 by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-P53 antibody A300-247A (lot A300-247A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human P53 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-P53 antibody A300-247A (lot A300-247A-2) used for IP at 3 µg per reaction. P53 was also immunoprecipitated by rabbit anti-P53 antibody A300-249A. For blotting immunoprecipitated P53, A300-247A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human p53 by immunohistochemistry. Sample: FFPE section of human lung cancer Antibody: Affinity purified rabbit anti- p53 (Cat. No. A300-247A Lot2) used at 1:5,000 (0.2µg/ml). Detection: DAB

Flow Cytometrical Analysis of p53 in Jurkat Cells. Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 X 10^6 cells were stained with 1.5 µg anti-KLH IgG control or anti-p53 [A300-247A] and secondary FITC-conjugated goat anti-rabbit (in a 150µl reaction). anti-KLH control IgG (black); anti-p53 (red).

Localization of p53 Binding Sites by ChIP-sequencing. Chromatin from MCF-7 was immunoprecipitated with anti-p53 antibody A300-247A and analyzed by DNA sequencing. The figure illustrates the peak distribution of p53 binding within a 250 Kb region of chromosome 6 as detected using anti-p53 A300-247A. ChIP-seq validation performed by Active Motif, Carlsbad, CA.

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