Antibody was affinity purified using an epitope specific to RPA32 immobilized on solid support. The epitope recognized by A300-244A maps to a region between residue 225 and the C-terminus (residue 270) of human Replication Protein A2 (32kD) using the numbering given in entry NP_002937.1 (GeneID 6118). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
REPA2, replication factor A protein 2, replication protein A 32 kDa subunit, replication protein A 34 kDa subunit, RF-A protein 2, RP-A p32, RP-A p34, RPA32
between 225 and C-term
2 - 8°C
Detection of human and mouse RPA32 by western blot. Samples: Whole cell lysate (5 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA32 antibody A300-244A (lot A300-244A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human RPA32 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with 100 µM etoposide for 4 hours (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-RPA32 antibody A300-244A (lot A300-244A-3) used for IP at 3 µg per reaction. RPA32 was also immunoprecipitated by rabbit anti-Phospho RPA32 (S4/S8) recombinant monoclonal antibody [BL-165-5F1] (A700-009). For blotting immunoprecipitated Phospho RPA32 (S4/S8), A700-009 was used at 1:1000. Chemiluminescence with an exposure time of 3 minutes.
Detection of human RPA32 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RPA32 (Cat. No. A300-244A Lot 3) used at a dilution of 1:5,000 (0.2 µg/ml). Detection: DAB