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Rabbit anti-RPA70 Antibody, Affinity Purified - A300-241A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-RPA70 Antibody, Affinity Purified
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Size: 100 µg
£302.00
GBP | Euro | USD
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Catalogue number:
A300-241A
Name:
Rabbit anti-RPA70 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to RPA70 immobilized on solid support. The epitope recognized by A300-241A maps to a region between residues 350 and 400 of human replication protein A1, 70kDa using the numbering given in entry NP_002936.1 (GeneID 6117).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP, IHC

Product Type:
Antibodies
Alternative Names:
HSSB, MST075, MSTP075, REPA1, replication factor A protein 1, replication protein A 70 kDa DNA-binding subunit, replication protein A1, 70kDa, RF-A, RF-A protein 1, RP-A, RP-A p70, RPA70, single-stranded DNA-binding protein
Species Reactivity:
Human, Mouse
Immunogen:
Between 350 and 400
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human RPA70 by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa and (15 and 50 µg) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA70 antibody A300-241A (lot A300-241A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of mouse RPA70 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA70 antibody A300-241A (lot A300-241A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human RPA70 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPA70 antibody A300-241A (lot A300-241A-3) used for IP at 6 µg per reaction. RPA70 was also immunoprecipitated by a previous lot of this antibody (lot A300-241A-2). For blotting immunoprecipitated RPA70, A300-241A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human RPA70 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibodies: Affinity purified rabbit anti- RPA70 Cat. No. A300-241A Lot2 used at a dilution of 1:1,000 (1 µg/ml) (left) and Cat. No. A300-241A Lot3 used at a dilution of 1:5,000 (0.2µg/ml (right). Detection: DAB

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Detection of mouse RPA70 by immunohistochemistry. Samples: FFPE serial sections of mouse teratoma. Antibodies: Affinity purified rabbit anti- RPA70 Cat. No. A300-241A Lot2 used at a dilution of 1:1,000 (1 µg/ml) (left) and Cat. No. A300-241A Lot3 used at a dilution of 1:5,000 (0.2µg/ml) (right). Detection: DAB

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