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Rabbit anti-RPA70 Antibody, Affinity Purified

Catalogue number:
A300-241A
Size:
100 µg
Product is available in:
  • UK
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Antibody was affinity purified using an epitope specific to RPA70 immobilized on solid support. The epitope recognized by A300-241A maps to a region between residues 350 and 400 of human replication protein A1, 70kDa using the numbering given in entry NP_002936.1 (GeneID 6117). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP, IHC
Product Type:

Antibodies

Alternative Names:

HSSB, MST075, MSTP075, REPA1, replication factor A protein 1, replication protein A 70 kDa DNA-binding subunit, replication protein A1, 70kDa, RF-A, RF-A protein 1, RP-A, RP-A p70, RPA70, single-stranded DNA-binding protein

Species Reactivity:

Human, Mouse

Immunogen:

between 350 and 400

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human RPA70 by western blot

Detection of human RPA70 by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa and (15 and 50 µg) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA70 antibody A300-241A (lot A300-241A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse RPA70 by western blot

Detection of mouse RPA70 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPA70 antibody A300-241A (lot A300-241A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human RPA70 by western blot

Detection of human RPA70 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPA70 antibody A300-241A (lot A300-241A-3) used for IP at 6 µg per reaction. RPA70 was also immunoprecipitated by a previous lot of this antibody (lot A300-241A-2). For blotting immunoprecipitated RPA70, A300-241A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human RPA70 by immunohistoc

Detection of human RPA70 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibodies: Affinity purified rabbit anti- RPA70 Cat. No. A300-241A Lot2 used at a dilution of 1:1,000 (1 µg/ml) (left) and Cat. No. A300-241A Lot3 used at a dilution of 1:5,000 (0.2µg/ml (right). Detection: DAB

Detection of mouse RPA70 by immunohistoc

Detection of mouse RPA70 by immunohistochemistry. Samples: FFPE serial sections of mouse teratoma. Antibodies: Affinity purified rabbit anti- RPA70 Cat. No. A300-241A Lot2 used at a dilution of 1:1,000 (1 µg/ml) (left) and Cat. No. A300-241A Lot3 used at a dilution of 1:5,000 (0.2µg/ml) (right). Detection: DAB