Antibody was affinity purified using an epitope specific to WRN immobilized on solid support. The epitope recognized by A300-238A maps to a region between residues 400 and 450 of human Werner Syndrome Helicase using the numbering given in SwissProt entry Q14191 (GeneID 7486). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DNA helicase, RecQ-like type 3, exonuclease WRN, recQ protein-like 2, RECQ3, RECQL2, RECQL3, Werner syndrome ATP-dependent helicase, Werner syndrome, RecQ helicase-like
between 400 and 450
2 - 8°C
Detection of human WRN by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WRN antibody A300-238A (lot A300-238A-2) used for WB at 0.2 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. WRN was also immunoprecipitated by rabbit anti-WRN antibody A300-239A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).