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Rabbit anti-WRN Antibody, Affinity Purified - A300-238A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-WRN Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-238A
Name:
Rabbit anti-WRN Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to WRN immobilized on solid support. The epitope recognized by A300-238A maps to a region between residues 400 and 450 of human Werner Syndrome Helicase using the numbering given in SwissProt entry Q14191 (GeneID 7486).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
DNA helicase, RecQ-like type 3, exonuclease WRN, recQ protein-like 2, RECQ3, RECQL2, RECQL3, Werner syndrome ATP-dependent helicase, Werner syndrome, RecQ helicase-like
Species Reactivity:
Human
Immunogen:
Between 400 and 450
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human WRN by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WRN antibody A300-238A (lot A300-238A-2) used for WB at 0.2 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. WRN was also immunoprecipitated by rabbit anti-WRN antibody A300-239A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

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