www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

Rabbit anti-WRN Antibody, Affinity Purified

Catalogue number:
A300-238A
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£322.00 Shipping is calculated in checkout

Antibody was affinity purified using an epitope specific to WRN immobilized on solid support. The epitope recognized by A300-238A maps to a region between residues 400 and 450 of human Werner Syndrome Helicase using the numbering given in SwissProt entry Q14191 (GeneID 7486). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

DNA helicase, RecQ-like type 3, exonuclease WRN, recQ protein-like 2, RECQ3, RECQL2, RECQL3, Werner syndrome ATP-dependent helicase, Werner syndrome, RecQ helicase-like

Species Reactivity:

Human

Immunogen:

between 400 and 450

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human WRN by western blot a

Detection of human WRN by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WRN antibody A300-238A (lot A300-238A-2) used for WB at 0.2 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. WRN was also immunoprecipitated by rabbit anti-WRN antibody A300-239A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).