Antibody was affinity purified using an epitope specific to SMC5 immobilized on solid support. The epitope recognized by A300-236A maps to a region between residues 1050 and the C-terminus (residue 1101) of human Structural Maintenance of Chromosomes 5 using the numbering given in TrEMBL entry Q8IY18 (GeneID 23137). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
hSMC5, SMC protein 5, SMC-5, SMC5 structural maintenance of chromosomes 5-like 1, SMC5L1, structural maintenance of chromosomes protein 5
between 1050 and C-term
2 - 8°C
Detection of human and mouse SMC5 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, Jurkat, TCMK-1 and NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-SMC5 antibody A300-236A (lot A300-236A-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human SMC5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-SMC5 antibody A300-236A (lot A300-236A-2) used for IP at 6 µg/mg lysate. SMC5 was also immunoprecipitated by a prior lot (A300-236A-1) of this antibody. For blotting immunoprecipitated SMC5, A300-236A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.