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Goat anti-SDS3 Antibody, Affinity Purified - A300-235A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Goat anti-SDS3 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-235A
Name:
Goat anti-SDS3 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to SDS3 immobilized on solid support. The epitope recognized by A300-235A maps to a region between residues 175 and 225 of human Suppressor of Defective Silencing 3 using the numbering given in entry NP_071936.2 (GeneID 64426).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
45 kDa Sin3-associated polypeptide, SAP45, SDS3, sin3 histone deacetylase corepressor complex component SDS3, sin3A-associated protein, 45kDa, suppressor of defective silencing 3 protein homolog
Species Reactivity:
Human, Mouse
Immunogen:
Between 175 and 225
Storage Temp:
2 - 8°C
Antibody Host:
Goat
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse SDS3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-SDS3 antibody A300-235A (lot A300-235A-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human SDS3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-SDS3 antibody A300-235A (lot A300-235A-2) used for IP at 6 µg per reaction. SDS3 was also immunoprecipitated by a previous lot of this antibody (A300-235A-1) and rabbit anti-SDS3 antibody A301-777A. For blotting immunoprecipitated SDS3, A300-235A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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