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Rabbit anti-KIF14 Antibody, Affinity Purified

Catalogue number:
A300-233A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to KIF14 immobilized on solid support. The epitope recognized by A300-233A maps to a region between residues 900 and 950 of human Kinesin Family Member 14 using the numbering given in entry NP_055690.1 (GeneID 9928). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

epididymis secretory sperm binding protein, kinesin-like protein KIF14, MCPH20, MKS12

Species Reactivity:

Human

Immunogen:

between 900 and 950

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human KIF14 by western blot

Detection of human KIF14 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KIF14 antibody A300-233A (lot A300-233A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human KIF14 by western blot

Detection of human KIF14 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-KIF14 antibody A300-233A (lot A300-233A-2) used for IP at 6 µg per reaction. KIF14 was also immunoprecipitated by a previous lot of this antibody (A300-233A-1) and rabbit anti-KIF14 antibody A300-232A. For blotting immunoprecipitated KIF14, A300-233A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.