Antibody was affinity purified using an epitope specific to KIF14 immobilized on solid support. The epitope recognized by A300-232A maps to a region between residues 50 and 100 of human Kinesin Family Member 14 using the numbering given in entry NP_055690.1 (GeneID 9928). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
epididymis secretory sperm binding protein, kinesin-like protein KIF14, MCPH20, MKS12
between 50 and 100
2 - 8°C
Detection of human KIF14 by western blot and immunoprecipitation. Samples: Whole cell lysate (WCL; 50 µg for input; 1 mg/IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-KIF14 antibodies BL357 (Cat. No. A300-232A) used for WB at 1 µg/ml and used for IP at 3 µg/mg lysate. KIF14 was also immunoprecipitated using rabbit anti-KIF14 antibody BL358 (Cat. No. A300-233A). Detection: Chemiluminescence with an exposure time of 10 seconds.