Antibody was affinity purified using an epitope specific to phosphorylation at Ser 485 and Thr 488 of XRCC1 immobilized on solid support. The epitope recognized by A300-231A maps to a region of surrounding residues Ser 485 and Thr 488 of human X-ray Repair Complementing protein 1 using the numbering given in SwissProt entry P18887 (GeneID 7515). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DNA repair protein XRCC1, RCC, SCAR26, X-ray repair complementing defective repair in Chinese hamster cells 1, X-ray repair cross-complementing protein 1
surrounding serine 485 and threonine 488
2 - 8°C
Detection of human and mouse Phospho-XRCC1-S485/T488 by western blot. Samples: Whole cell lysate (50 µg) from asynchronously growing HeLa (H), HEK293T (T) and mouse NIH-3T3 (M) cells. Antibodies: Affinity purified rabbit anti-Phospho-XRCC1-S485/T488 antibody A300-231A (lot A300-231A-2) used for WB at 0.1 µg/ml. The binding of the antibody was blocked by pre-incubation with the phospho-peptide but was not blocked by pre-incubation with the non-phosphorylated peptide. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human and mouse Phospho-XRCC1-S485/T488 by immunohistochemistry. Sample: FFPE sections of human stomach carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- Phospho-XRCC1-S485/T488 (Cat. No. A300-231A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB