www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

Rabbit anti-Phospho XRCC1 (S485/T488) Antibody, Affinity Purified

Catalogue number:
A300-231A
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£343.00 Shipping is calculated in checkout

Antibody was affinity purified using an epitope specific to phosphorylation at Ser 485 and Thr 488 of XRCC1 immobilized on solid support. The epitope recognized by A300-231A maps to a region of surrounding residues Ser 485 and Thr 488 of human X-ray Repair Complementing protein 1 using the numbering given in SwissProt entry P18887 (GeneID 7515). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IHC
Product Type:

Antibodies

Alternative Names:

DNA repair protein XRCC1, RCC, SCAR26, X-ray repair complementing defective repair in Chinese hamster cells 1, X-ray repair cross-complementing protein 1

Species Reactivity:

Human, Mouse

Immunogen:

surrounding serine 485 and threonine 488

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse Phospho-XRC

Detection of human and mouse Phospho-XRCC1-S485/T488 by western blot. Samples: Whole cell lysate (50 µg) from asynchronously growing HeLa (H), HEK293T (T) and mouse NIH-3T3 (M) cells. Antibodies: Affinity purified rabbit anti-Phospho-XRCC1-S485/T488 antibody A300-231A (lot A300-231A-2) used for WB at 0.1 µg/ml. The binding of the antibody was blocked by pre-incubation with the phospho-peptide but was not blocked by pre-incubation with the non-phosphorylated peptide. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human and mouse Phospho-XRC

Detection of human and mouse Phospho-XRCC1-S485/T488 by immunohistochemistry. Sample: FFPE sections of human stomach carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- Phospho-XRCC1-S485/T488 (Cat. No. A300-231A Lot2) used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB