Antibody was affinity purified using an epitope specific to SMARCA3 immobilized on solid support. The epitope recognized by A300-229A maps to a region between residues 900 and 950 of human SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily a, member 3 using the numbering given in entry NP_620636.1 (GeneID 6596). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DNA-binding protein/plasminogen activator inhibitor 1 regulator, DNA-binding protein/plasminogen activator inhibitor-1 regulator, helicase-like transcription factor, HIP116, HIP116A, HLTF1, RING finger protein 80, RING-type E3 ubiquitin transferase HLTF,
between 900 and 950
2 - 8°C
Detection of human SMARCA3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCA3 antibody A300-229A (lot A300-229A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human SMARCA3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMARCA3 antibody A300-229A (lot A300-229A-2) used for IP at 6 µg per reaction. SMARCA3 was also immunoprecipitated by rabbit anti-SMARCA3 antibody A300-230A. For blotting immunoprecipitated SMARCA3, A300-230A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.