www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

Rabbit anti-SMARCA3 Antibody, Affinity Purified

Catalogue number:
A300-229A
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£322.00 Shipping is calculated in checkout

Antibody was affinity purified using an epitope specific to SMARCA3 immobilized on solid support. The epitope recognized by A300-229A maps to a region between residues 900 and 950 of human SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily a, member 3 using the numbering given in entry NP_620636.1 (GeneID 6596). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

DNA-binding protein/plasminogen activator inhibitor 1 regulator, DNA-binding protein/plasminogen activator inhibitor-1 regulator, helicase-like transcription factor, HIP116, HIP116A, HLTF1, RING finger protein 80, RING-type E3 ubiquitin transferase HLTF,

Species Reactivity:

Human

Immunogen:

between 900 and 950

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Related Content:
Detection of human SMARCA3 by western bl

Detection of human SMARCA3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMARCA3 antibody A300-229A (lot A300-229A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human SMARCA3 by western bl

Detection of human SMARCA3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMARCA3 antibody A300-229A (lot A300-229A-2) used for IP at 6 µg per reaction. SMARCA3 was also immunoprecipitated by rabbit anti-SMARCA3 antibody A300-230A. For blotting immunoprecipitated SMARCA3, A300-230A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.