Antibody was affinity purified using an epitope specific to HELLS immobilized on solid support. The epitope recognized by A300-226A maps to a region between residues 550 and 600 of human Helicase, Lymphoid-Specific using the numbering given in entry NP_060533.2 (GeneID 3070). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ICF4, LSH, lymphoid-specific helicase, Nbla10143, PASG, proliferation-associated SNF2-like protein, SMARCA6, SWI/SNF2-related matrix-associated actin-dependent regulator of chromatin subfamily A member 6, SWI/SNF2-related, matrix-associated, actin-depende
between 550 and 600
2 - 8°C
Detection of human HELLS by western blot. Samples: Whole cell lysate (50 µg) from HeLa and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HELLS antibody A300-226A (lot A300-226A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.