Antibody was affinity purified using an epitope specific to Cul7 immobilized on solid support. The epitope recognized by A300-224A maps to a region between residues 1650 and the C-terminus (residue 1698) of human Cullin homolog 7 using the numbering given in SwissProt entry Q14999 (GeneID 9820). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
3M1, CUL-7, cullin-7, dJ20C7.5, KIAA0076
between 1650 and C-term
2 - 8°C
Detection of human Cul7 by western blot and immunoprecipitation. Samples: Whole cell lysate (1 mg for IP) from U2OS cells. Antibodies: Affinity purified rabbit anti-Cul7 antibody BL654 (Cat. No. A300-224A) at 1 and 5 µg/mg lysate for IP. Cul-7 was also immunoprecipitated using rabbit anti-Cul7 antibody BL653 (Cat. No. A300-223A). Cul7 was detected by WB using BL653 at 1 µg/ml. immunoprecipitation was also performed using normal rabbit IgG (control IP) Detection: Chemiluminescence with exposure times of 10 seconds.