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Rabbit anti-Cul7 Antibody, Affinity Purified

Catalogue number:
A300-223A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to Cul7 immobilized on solid support. The epitope recognized by A300-223A maps to a region between residues 1500 and 1550 of human Cullin homolog 7 using the numbering given in SwissProt entry Q14999 (GeneID 9820). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

3M1, CUL-7, cullin-7, dJ20C7.5, KIAA0076

Species Reactivity:

Human, Mouse

Immunogen:

between 1500 and 1550

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse Cul7 by wes

Detection of human and mouse Cul7 by western blot. Samples: Whole cell lysate (50 µg) from Hep-G2, HEK293T, HeLa, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cul7 antibody A300-223A (lot A300-223A-4) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Cul7 by western blot

Detection of human Cul7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cul7 antibody A300-223A (lot A300-223A-4) used for IP at 6 µg per reaction. Cul7 was also immunoprecipitated by a previous lot of this antibody (lot A300-223A-3) and rabbit anti-Cul7 antibody A300-224A. For blotting immunoprecipitated Cul7, A300-223A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.