Antibody was affinity purified using an epitope specific to DMAP1 immobilized on solid support. The epitope recognized by A300-218A maps to a region between residues 400 and 450 of human DNA Methyltransferase 1 Associated Protein 1 using the numbering given in entry NP_061973.1 (GeneID 55929). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DNA methyltransferase 1-associated protein 1, DNMAP1, DNMT1 associated protein 1, DNMT1-associated protein 1, DNMTAP1, EAF2, MEAF2, SWC4
between 400 and 450
2 - 8°C
Detection of human DMAP1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DMAP1 antibody A300-218A (lot A300-218A-2) used for WB at 0.5 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.