Rabbit anti-FKBP-135 Antibody, Affinity Purified - A300-210A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to FKBP-135 immobilized on solid support. The epitope recognized by A300-210A maps to a region between residues 960 and 1020 of human FK506-binding protein 135 using the numbering given in TrEMBL entry Q9Y4D0 (GeneID 23307). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP
Detection of human FKPB-135 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FKPB-135 antibody A300-210A (lot A300-210A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human FKPB-135 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FKPB-135 antibody A300-210A (lot A300-210A-2) used for IP at 6 µg per reaction. FKPB-135 was also immunoprecipitated by rabbit anti-FKPB-135 antibody A300-109A. For blotting immunoprecipitated FKPB-135, A300-210A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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