Antibody was affinity purified using an epitope specific to KIAA1276 immobilized on solid support. The epitope recognized by A300-208A maps to a region between residue 1000 and the C-terminus (residue 1068) of human KIAA1276 using the numbering given in TrEMBL entry Q9ULE4 (GeneID 27146). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
between 1000 and C-term
2 - 8°C
Detection of human KIAA1276 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KIAA1276 antibody A300-208A (lot A300-208A-1) used for WB at 0.066 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.