Antibody was affinity purified using an epitope specific to Pumilio 2 immobilized on solid support. The epitope recognized by A300-202A maps to a region between residues 100 and 150 of human Pumilio 2 using the numbering given in entry NP_056132.1 (GeneID 23369). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
PUMH2, pumilio homolog 2, pumilio-2, PUML2
between 100 and 150
2 - 8°C
Detection of human and mouse Pumilio 2 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Pumilio 2 antibody A300-202A (lot A300-202A-2) used for WB at 0.2 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).