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Goat anti-Pumilio 1 Antibody, Affinity Purified - A300-201A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Goat anti-Pumilio 1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-201A
Name:
Goat anti-Pumilio 1 Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Pumilio immobilized on solid support. The epitope recognized by A300-201A maps to a region between residues 225 and 275 of human Pumilio 1 using the numbering given in entry NP_055491.1 (GeneID 9698).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
HSPUM, PUMH, PUMH1, pumilio homolog 1, pumilio-1, PUML1, SCA47
Species Reactivity:
Human, Mouse
Immunogen:
Between 225 and 275
Storage Temp:
2 - 8°C
Antibody Host:
Goat
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human and mouse Pumillio 1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pumillio 1 antibody A300-201A (lot A300-201A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human Pumillio 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pumillio 1 antibody A300-201A (lot A300-201A-2) used for IP at 6 µg per reaction. Pumillio 1 was also immunoprecipitated by a previous lot of this antibody (lot A301-201A-1). For blotting immunoprecipitated Pumillio 1, A300-201A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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