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Goat anti-Pumilio 1 Antibody, Affinity Purified

Catalogue number:
A300-201A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to Pumilio immobilized on solid support. The epitope recognized by A300-201A maps to a region between residues 225 and 275 of human Pumilio 1 using the numbering given in entry NP_055491.1 (GeneID 9698). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

HSPUM, PUMH, PUMH1, pumilio homolog 1, pumilio-1, PUML1, SCA47

Species Reactivity:

Human, Mouse

Immunogen:

between 225 and 275

Storage Temp:

2 - 8°C

Antibody Host:

Goat

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human and mouse Pumillio 1

Detection of human and mouse Pumillio 1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pumillio 1 antibody A300-201A (lot A300-201A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Pumillio 1 by western

Detection of human Pumillio 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pumillio 1 antibody A300-201A (lot A300-201A-2) used for IP at 6 µg per reaction. Pumillio 1 was also immunoprecipitated by a previous lot of this antibody (lot A301-201A-1). For blotting immunoprecipitated Pumillio 1, A300-201A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.