Antibody was affinity purified using an epitope specific to CKII alpha’ immobilized on solid support. The epitope recognized by A300-199A maps to a region between residue 300 and the C-terminus (residue 350) of human Casein Kinase 2, alpha 2 using the numbering given in entry NP_001887.1 (GeneID 1459). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
casein kinase 2 alpha', casein kinase 2, alpha prime polypeptide, casein kinase II subunit alpha', CK II alpha', CK2A2, CK2alpha', CSNK2A1
between 300 and C-term
2 - 8°C
Detection of human and mouse CKII alpha' by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha' antibody A300-199A (lot A300-199A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human CKII alpha' by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CKII alpha' antibody A300-199A (lot A300-199A-5) used for IP at 6 µg per reaction. CKII alpha' was also immunoprecipitated by rabbit anti-CKII alpha' antibody A300-198A. For blotting immunoprecipitated CKII alpha', A300-199A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.