Rabbit anti-CKII alpha' Antibody, Affinity Purified

Name: Rabbit anti-CKII alpha' Antibody, Affinity Purified
Brand: Bethyl Laboratories, Inc.
SKU: CBSKU_00070497
Price: 436 GBP
Availability: InStock

Catalogue number:

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A300-199A

Supplier:

Bethyl Laboratories, Inc.

Size:

100 µl (1000 µg/ml) _$$_

Product is available in:

Currency:
GBP
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£436.00

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Estimated delivery Fri 26 Apr
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Applications:

WB, IP

Antibody Host:

Rabbit

Target:

CKII alpha

Species Reactivity:

Human; Mouse

Antibody Type:

Polyclonal

Immunogen:

between 300 and C-term

Alternative Names:

casein kinase II subunit alpha'; CSNK2A1; CK2alpha'; CK II alpha'; casein kinase 2, alpha prime polypeptide; casein kinase 2 alpha'; CK2A2

Product Description:

Rabbit anti-CKII alpha' Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human and mouse CKII alpha' by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha' antibody A300-199A (lot A300-199A-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human CKII alpha' by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CKII alpha' antibody A300-199A (lot A300-199A-5) used for IP at 6 µg per reaction. CKII alpha' was also immunoprecipitated by rabbit anti-CKII alpha' antibody A300-198A. For blotting immunoprecipitated CKII alpha', A300-199A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

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