Rabbit anti-MCM5 Antibody, Affinity Purified - A300-195A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to MCM5 immobilized on solid support. The epitope recognized by A300-195A maps to a region between residues 1 and 50 of human Minichromosome Maintenance 5 using the numbering given in entry NP_006730.2 (GeneID 4174). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, IHC
Detection of human MCM5 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM5 antibody A300-195A (lot A300-195A-2) used for WB at 0.05 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human and mouse MCM5 by immunohistochemistry. Sample: FFPE sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- MCM5 (Cat. No. A300-195A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
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