Antibody was affinity purified using an epitope specific to MCM4 immobilized on solid support. The epitope recognized by A300-193A maps to a region between residues 750 and 800 of human Minichromosome Maintenance 4 using the numbering given in entry NP_005905.2 (GeneID 4173). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. .
CDC21, CDC21 homolog, CDC54, DNA replication licensing factor MCM4, hCdc21, homolog of S. pombe cell devision cycle 21, IMD54, minichromosome maintenance deficient 4, NKCD, NKGCD, P1-CDC21
between 750 and 800
2 - 8°C
Detection of human MCM4 by western blot. Sample: RIPA extract (50 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-MCM4 (Cat. No. A300-193A) used at 0.2 µg/ml. Detection: Chemiluminescence.
Detection of human and mouse MCM4 by immunohistochemistry. Sample: FFPE section of human testicular seminoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti-MCM4 (Cat. No. A300-193A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB