Antibody was affinity purified using an epitope specific to MCM3 immobilized on solid support. The epitope recognized by A300-192A maps to a region between residues 500 and 550 of human Minichromosome Maintenance 3 using the numbering given in entry NP_002379.2 (GeneID 4172). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
cervical cancer proto-oncogene 5, DNA polymerase alpha holoenzyme-associated protein P1, DNA replication factor MCM3, DNA replication licensing factor MCM3, HCC5, hRlf beta subunit, MCM3 minichromosome maintenance deficient 3, minichromosome maintenance d
between 500 and 550
2 - 8°C
Detection of human MCM3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM3 antibody A300-192A (lot A300-192A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.