Antibody was affinity purified using an epitope specific to RFC3 immobilized on solid support. The epitope recognized by A300-188A maps to a region between residues 80 and 110 of human replication factor C (Activator 1) 3, 38kDa using the numbering given in SwissProt entry P40938 (GeneID 5983). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
A1 38 kDa subunit, activator 1 38 kDa subunit, activator 1 subunit 3, replication factor C (activator 1) 3, 38kDa, replication factor C 38 kDa subunit, replication factor C subunit 3, RF-C 38 kDa subunit, RFC, 38 kD subunit, RFC38
between 80 and 110
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human RFC3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFC3 antibody A300-188A (lot A300-188A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.