Rabbit anti-RFC3 Antibody, Affinity Purified - A300-188A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to RFC3 immobilized on solid support. The epitope recognized by A300-188A maps to a region between residues 80 and 110 of human replication factor C (Activator 1) 3, 38kDa using the numbering given in SwissProt entry P40938 (GeneID 5983).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IHC
Detection of human RFC3 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFC3 antibody A300-188A (lot A300-188A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human RFC3 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- RFC3 (Cat. No. A300-188A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
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