Antibody was affinity purified using an epitope specific to Rad50 immobilized on solid support. The epitope recognized by A300-184A maps to a region between residues 50 and 100 of human Rad50 using the numbering given in entry NP_005723.2 (GeneID 10111). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DNA repair protein RAD50, hRad50, NBSLD, RAD50 homolog, double strand break repair protein, RAD502
between 50 and 100
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human Rad50 by immunoprecipitation and western blot. Sample: Nuclear extract (1 mg) from HeLa cells. Antibodies: Affinity purified rabbit anti-Rad50 (Cat. No. A300-184A) used at 0.25 µg/ml for WB of Rad50 immunoprecipitated by 1.25 µg of affinity purified rabbit anti-Rad50 (BL190) or affinity purified rabbit anti-Rad50 (BL193; Cat. No. A300-185A). Detection: Chemiluminescence.