Antibody was affinity purified using an epitope specific to QKI immobilized on solid support. The epitope recognized by A300-182A maps to a region between residues 50 and 100 of isoform 5 of human quaking homolog using the numbering given in entry AAH19917.1 (GeneID 9444). The epitope is common to isoforms 1, 2, 3, 4, 5, 6, 7 and 7B according to TrEMBL entries Q9P0Y1, Q9P0Y0, Q9P0X6, Q9P0X8, Q96PU8, Q96PU7, Q969L9 and Q96PU6, respectively. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
homolog of mouse quaking QKI (KH domain RNA binding protein), Hqk, hqkI, protein quaking, QK, QK1, QK3, QKI/LOC100132735 fusion, quaking homolog, KH domain RNA binding, RNA binding protein HQK
between 50 and 100
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human QKI by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-QKI antibody A300-182A (lot A300-182A-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.