Antibody was affinity purified using an epitope specific to Mre11a immobilized on solid support. The epitope recognized by A300-181A maps to a region between residues 1 and 50 of human Meiotic Recombination 11 homolog A using the numbering given in entry NP_005582.1 (GeneID 4361). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATLD, AT-like disease, DNA recombination and repair protein, double-strand break repair protein MRE11, double-strand break repair protein MRE11A, endo/exonuclease Mre11, HNGS1, meiotic recombination 11 homolog 1, meiotic recombination 11 homolog A, MRE11
between 1 and 50
2 - 8°C
Detection of human Mre11a by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Mre11a antibody A300-181A (lot A300-181A-3) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.