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Rabbit anti-Rtf1 Antibody, Affinity Purified

Catalogue number:
A300-179A
Size:
100 µg
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Antibody was affinity purified using an epitope specific to Rtf1 immobilized on solid support. The epitope recognized by A300-179A maps to a region between residue 620 and 670 of human Restores TBP (TATA box-binding protein) function-1 using the numbering given in entry EAW92492.1 (GeneID 23168). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

GTL7, KIAA0252, ortholog of mouse gene trap locus 7, RNA polymerase-associated protein RTF1 homolog, Rtf1, Paf1/RNA polymerase II complex component, homolog

Species Reactivity:

Human

Immunogen:

between 620 and 670

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Rtf1 by western blot.

Detection of human Rtf1 by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rtf1 antibody A300-179A (lot A300-179A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Rtf1 by western blot

Detection of human Rtf1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rtf1 antibody A300-179A (lot A300-179A-1) used for IP at 3 µg per reaction. Rtf1 was also immunoprecipitated by rabbit anti-RTF1 antibodies A300-178A and A301-329A. For blotting immunoprecipitated Rtf1, A300-179A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.