Antibody was affinity purified using an epitope specific to Rtf1 immobilized on solid support. The epitope recognized by A300-178A maps to a region between residues 300 and 350 of human Restores TBP (TATA box-binding protein) function-1 using the numbering given in entry EAW92492.1 (GeneID 23168). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
GTL7, KIAA0252, ortholog of mouse gene trap locus 7, RNA polymerase-associated protein RTF1 homolog, Rtf1, Paf1/RNA polymerase II complex component, homolog
between 300 and 350
2 - 8°C
Detection of human RTF1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RTF1 antibody A300-178A used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. RTF1 was successfully immunoprecipitated by rabbit anti-RTF1 antibodies A300-179A and A301-329A, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).