Antibody was affinity purified using an epitope specific to Leo1 immobilized on solid support. The epitope recognized by A300-174A maps to a region between residues 350 and 400 of human senescence downregulated leo1-like using the numbering given in TrEMBL entry Q8WVC0 (GeneID 123169). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Leo1 Paf1/RNA polymerase II complex component, Leo1, Paf1/RNA polymerase II complex component, homolog, RDL, replicative senescence down-regulated leo1-like protein, RNA polymerase-associated protein LEO1
between 350 and 400
2 - 8°C
Detection of human and mouse Leo1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Leo1 antibody A300-174A (lot A300-174A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.