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Rabbit anti-Paf1 Antibody, Affinity Purified - A300-173A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Paf1 Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-173A
Name:
Rabbit anti-Paf1 Antibody, Affinity Purified
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Availability:

Antibody was affinity purified using an epitope specific to Paf1 immobilized on solid support. The epitope recognized by A300-173A maps to a region between residues 475 and 525 of human RNA Polymerase Associated Factor using the numbering given in TrEMBL entry Q9NUU9 (GeneID 54623).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

IP, IHC

Product Type:
Antibodies
Alternative Names:
F23149_1, hPAF1, Paf1, RNA polymerase II associated factor, homolog, pancreatic differentiation protein 2, PD2, RNA polymerase II-associated factor 1 homolog
Species Reactivity:
Human, Mouse
Immunogen:
Between 475 and 525
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Paf1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Paf1 antibody A300-173A (lot A300-173A-3) used for IP at 6 µg per reaction. Paf1 was also immunoprecipitated by a previous lot of this antibody (lot A300-173A-2) and rabbit anti-Paf1 antibody A300-172A-2 For blotting immunoprecipitated Paf1, A300-173A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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Detection of human Paf1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Paf1 (Cat. No. A300-173A Lot3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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Detection of mouse Paf1 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- Paf1 (Cat. No. A300-173A Lot3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

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