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Rabbit anti-Paf1 Antibody, Affinity Purified

Catalogue number:
A300-173A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to Paf1 immobilized on solid support. The epitope recognized by A300-173A maps to a region between residues 475 and 525 of human RNA Polymerase Associated Factor using the numbering given in TrEMBL entry Q9NUU9 (GeneID 54623). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: IP, IHC
Product Type:

Antibodies

Alternative Names:

F23149_1, hPAF1, Paf1, RNA polymerase II associated factor, homolog, pancreatic differentiation protein 2, PD2, RNA polymerase II-associated factor 1 homolog

Species Reactivity:

Human, Mouse

Immunogen:

between 475 and 525

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Paf1 by western blot

Detection of human Paf1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Paf1 antibody A300-173A (lot A300-173A-3) used for IP at 6 µg per reaction. Paf1 was also immunoprecipitated by a previous lot of this antibody (lot A300-173A-2) and rabbit anti-Paf1 antibody A300-172A-2 For blotting immunoprecipitated Paf1, A300-173A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Paf1 by immunohistoch

Detection of human Paf1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Paf1 (Cat. No. A300-173A Lot3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

Detection of mouse Paf1 by immunohistoch

Detection of mouse Paf1 by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- Paf1 (Cat. No. A300-173A Lot3) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB