Antibody was affinity purified using an epitope specific to Paf1 immobilized on solid support. The epitope recognized by A300-172A maps to a region between residues 75 and 125 of human RNA Polymerase Associated Factor using the numbering given in TrEMBL entry Q9NUU9 (GeneID 54623). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
F23149_1, hPAF1, Paf1, RNA polymerase II associated factor, homolog, pancreatic differentiation protein 2, PD2, RNA polymerase II-associated factor 1 homolog
between 75 and 125
2 - 8°C
Detection of human and mouse Paf1 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Paf1 antibody A300-172A (lot A300-172A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human Paf1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Paf1 antibody A300-172A (lot A300-172A-3) used for IP at 3 µg per reaction. Paf1 was also immunoprecipitated by rabbit anti-Paf1 antibody A300-173A. For blotting immunoprecipitated Paf1, A300-172A was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.