Antibody was affinity purified using an epitope specific to MCAF immobilized on solid support. The epitope recognized by A300-169A maps to a region between residues 25 and 100 of human MBD1-containing Chromatin Associated Factor using the numbering given in entry AAQ92978.1 (GeneID 55729). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
activating transcription factor 7-interacting protein 1, AM, ATF7-interacting protein, ATFa-associated modulator, ATF-interacting protein, ATF-IP, hAM, MBD1-containing chromatin-associated factor 1, MCAF, MCAF1, p621
between 25 and 100
2 - 8°C
Detection of human MCAF by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCAF antibody A300-169A (lot A300-169A-1) used for WB at 0.05 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human MCAF by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MCAF (Cat. No. A300-169A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB