Antibody was affinity purified using an epitope specific to ELYS immobilized on solid support. The epitope recognized by A300-166A maps to a region between residues 2220 and the C-terminus (residue 2302) of human ELYS transcription factor-like protein TMBS62 using the number given in entry AAN65622.1 (GeneID 25909) Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ELYS, ELYS transcription factor-like protein TMBS62, embryonic large molecule derived from yolk sac, MST108, MSTP108, protein ELYS, Protein MEL-28, putative AT-hook-containing transcription factor 1, TMBS62
between 2220 and C-term
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human ELYS by western blot and immunoprecipitation. Samples: Nuclear Extract (10 and 20 µg for WB; 1 mg for IP/WB) from HeLa cells. Antibodies: A) Affinity purified rabbit anti-ELYS antibody BL476 (Cat. No. A300-166A) used at 0.5 µg/ml for WB and 4 µg/mg lysate for IP. B) Affinity purified rabbit anti-ELYS antibodies BL476 and BL475 (Cat. No. A300-165A) were each used at 4 µg/mg extract for IP. immunoprecipitatesd ELYS was detected by WB using BL475 at 0.2 µg/ml. Detection: Chemiluminescence with exposure times of 3 to 5 minutes.