Antibody was affinity purified using an epitope specific to SA2 immobilized on solid support. The epitope recognized by A300-159A maps to a region between residues 1125 and 1175 of human Stromal Antigen 2 using the numbering given in entry NP_006594.3 (GeneID 10735). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
bA517O1.1, cohesin subunit SA-2, SA2, SA-2, SCC3 homolog 2, SCC3B, Stromal antigen 2
between 1125 and 1175
2 - 8°C
Detection of human and mouse SA2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SA2 antibody A300-159A (lot A300-159A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human SA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SA2 antibody A300-159A (lot A300-159A-2) used for IP at 6 µg per reaction. SA2 was also immunoprecipitated by rabbit anti-SA2 antibodies A302-581A and A301-582A. For blotting immunoprecipitated SA2, A300-159A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.