Goat anti-SA1 Antibody, Affinity Purified - A300-157A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to SA1 immobilized on solid support. The epitope recognized by A300-157A maps to a region between residues 1150 and 1200 of human Stromal Antigen 1 using the numbering given in entry NP_005853.2 (GeneID 10274). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB, IP, ICC
Detection of human SA1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SA1 antibody A300-157A (lot A300-157A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human SA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SA1 antibody A300-157A (lot A300-157A-3) used for IP at 6 µg per reaction. SA1 was also immunoprecipitated by a previous lot of this antibody (lot A300-157A-2). For blotting immunoprecipitated SA1, A300-157A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Localization of human SA1. Sample: HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody: Affinity purified goat anti-SA1 (BL143G; A300-157A) used at 1 µg/ml.
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