Antibody was affinity purified using an epitope specific to ZCWCC1 immobilized on solid support. The epitope recognized by A300-149A maps to a region between residues 600 and 700 of human zinc finger, CW-type with coiled-coil domain 1 using the numbering given in entry NP_055756.1 (GeneID 22880). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
CMT2Z, MORC family CW-type zinc finger protein 2, ZCW3, ZCWCC1, zinc finger CW-type coiled-coil domain protein 1
between 600 and 700
2 - 8°C
Detection of human ZCWCC1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZCWCC1 antibody A300-149A (lot A300-149A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human ZCWCC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZCWCC1 antibody A300-149A (lot A300-149A-2) used for IP at 3 µg per reaction. ZCWCC1 was also immunoprecipitated by rabbit anti-ZCWCC1 antibody A300-150A. For blotting immunoprecipitated ZCWCC1, A300-149A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.