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Rabbit anti-Aprataxin Antibody, Affinity Purified - A300-148A - 100 µg - Bethyl Laboratories, Inc. - antibodies

Rabbit anti-Aprataxin Antibody, Affinity Purified
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Size: 100 µg
£302.00
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Catalogue number:
A300-148A
Name:
Rabbit anti-Aprataxin Antibody, Affinity Purified
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Antibody was affinity purified using an epitope specific to Apratraxin immobilized on solid support. The epitope recognized by A300-148A maps to a region between residues 100 and 150 of human Aprataxin using the numbering given in SwissProt Entry Q7Z2E3 (GeneID 54840).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Applications:

WB, IP

Product Type:
Antibodies
Alternative Names:
AOA, AOA1, aprataxin, AXA1, EAOH, EOAHA, FHA-HIT, forkhead-associated domain histidine triad-like protein
Species Reactivity:
Human
Immunogen:
Between 100 and 150
Storage Temp:
2 - 8°C
Antibody Host:
Rabbit
Antibody Isotype:
Whole IgG
Antibody Type:
Polyclonal
UNSPSC Code:
12352203
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Detection of human Aprataxin by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aprataxin antibody A300-148A (lot A300-148A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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Detection of human Aprataxin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Aprataxin antibody A300-148A (lot A300-148A-2) used for IP at 6 µg/mg lysate. Aprataxin was also immunoprecipitated by a previous lot of this antibody (lot A300-148A-1). For blotting immunoprecipitated Aprataxin, A300-148A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

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