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Rabbit anti-Aprataxin Antibody, Affinity Purified

Catalogue number:
A300-148A
Size:
100 µg
Product is available in:
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Antibody was affinity purified using an epitope specific to Apratraxin immobilized on solid support. The epitope recognized by A300-148A maps to a region between residues 100 and 150 of human Aprataxin using the numbering given in SwissProt Entry Q7Z2E3 (GeneID 54840). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

AOA, AOA1, aprataxin, AXA1, EAOH, EOAHA, FHA-HIT, forkhead-associated domain histidine triad-like protein

Species Reactivity:

Human

Immunogen:

between 100 and 150

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human Aprataxin by western

Detection of human Aprataxin by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aprataxin antibody A300-148A (lot A300-148A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Aprataxin by western

Detection of human Aprataxin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Aprataxin antibody A300-148A (lot A300-148A-2) used for IP at 6 µg/mg lysate. Aprataxin was also immunoprecipitated by a previous lot of this antibody (lot A300-148A-1). For blotting immunoprecipitated Aprataxin, A300-148A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.