Antibody was affinity purified using an epitope specific to phosphorylation at Ser 461 of XRCC1 immobilized on solid support. The epitope recognized by A300-147A maps to a region of surrounding residue Ser 416 of human X-ray Repair Complementing protein 1 using the numbering given in SwissProt entry P18887 (GeneID 7515). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
DNA repair protein XRCC1, RCC, SCAR26, X-ray repair complementing defective repair in Chinese hamster cells 1, X-ray repair cross-complementing protein 1
surrounding serine 461
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human Phospho XRCC1 (S461) by immunohistochemistry. Samples: FFPE serial sections of human larynx squamous cell carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho XRCC1 (S461). (Cat. No. A300-147A Lot1) used at a dilution of 1:1,000 ( 1µg/ml. Detection: DAB