Antibody was affinity purified using an epitope specific to RFC5 immobilized on solid support. The epitope recognized by A300-146A maps to a region between residues 1 and 50 of human Replication Factor C subunit 5 using the numbering given in entry NP_031396.1 (GeneID 5985). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
A1 36 kDa subunit, Activator 1 36 kDa subunit, Activator 1 subunit 5, replication factor C (activator 1) 5, 36.5kDa, Replication factor C 36 kDa subunit, replication factor C subunit 5, replication factor C, 36-kDa subunit, RF-C 36 kDa subunit, RFC, 36.5
between 1 and 50
2 - 8°C
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Detection of human RFC5 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC5 antibody A300-146A (lot A300-146A-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.