Goat anti-RFC5 Antibody, Affinity Purified - A300-146A - 100 µg - Bethyl Laboratories, Inc. - antibodies
Antibody was affinity purified using an epitope specific to RFC5 immobilized on solid support. The epitope recognized by A300-146A maps to a region between residues 1 and 50 of human Replication Factor C subunit 5 using the numbering given in entry NP_031396.1 (GeneID 5985).Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Applications:
WB
Detection of human RFC5 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-RFC5 antibody A300-146A (lot A300-146A-2) used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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