Antibody was affinity purified using an epitope specific to RFC2 immobilized on solid support. The epitope recognized by A300-142A maps to a region between residues 1 and 50 of human Replication Factor C subunit 2 using the numbering given in entry NP_852136.1 (GeneID 5982). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
A1 40 kDa subunit, Activator 1 40 kDa subunit, activator 1 subunit 2, replication factor C (activator 1) 2, 40kDa, Replication factor C 40 kDa subunit, replication factor C subunit 2, RF-C 40 kDa subunit, RFC40
between 1 and 50
2 - 8°C
Detection of human RFC2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFC2 antibody A300-142A (lot A300-142A-1) used for WB at 0.2 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human RFC2 (aka RFC40) by western blot. Samples: Whole cell extract (50 µg) from MCF-7 and HEK293T cells (A) or nuclear extract (50 µg) from HeLa cells (B). Antibody: Affinity purified goat anti-RFC2 (Cat. No. A300-142A) used at 0.5 µg/ml (A) and 1 µg/ml (B). Detection: Chemiluminescence.